EVALUATION OF AN INDIRECT FLUORESCENT ANTIBODY TEST (IFAT) FOR DEMONSTRATION OF ANTIBODIES TO TOXOPLASMA GONDII IN THE SEA OTTER (ENHYDRA LUTRIS)

2002 ◽  
Vol 88 (3) ◽  
pp. 594-599 ◽  
Author(s):  
M. A. Miller ◽  
I. A. Gardner ◽  
A. Packham ◽  
J. K. Mazet ◽  
K. D. Hanni ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Sea Otter ◽  
Indirect Fluorescent Antibody ◽  
Enhydra Lutris

Investigation of Anti-Toxoplasma gondii Antibodies in Cats of the Ankara Region of Turkey Using the Sabin-Feldman Dye Test and an Indirect Fluorescent Antibody Test

2008 ◽  
Vol 94 (4) ◽  
pp. 817-820 ◽  
Author(s):  
Ayşegül Taylan Özkan ◽  
Bekir Çelebi ◽  
Cahit Babür ◽  
Araceli Lucio-Forster ◽  
Dwight D. Bowman ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Indirect Fluorescent Antibody

scholarly journals Seroprevalence of Toxoplasma gondii infection in swine matrices in Nova Mutum and Diamantino, Mato Grosso, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 254-255 ◽  
Author(s):  
Lívia Saab Muraro ◽  
João Garcia Caramori Júnior ◽  
Maria Regina Reis Amendoeira ◽  
Joyce Alves Pereira ◽  
João Xavier de Oliveira Filho ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
The State ◽  
Indirect Fluorescent Antibody ◽  
Risk Of Infection ◽  
Serum Samples ◽  
Mato Grosso ◽  
Toxoplasma Gondii Infection

This report aimed to assess the seroprevalence of Toxoplasma gondii infection in 708 swine matrices in Nova Mutum and Diamantino in the state of Mato Grosso, Central-West Brazil. Serum samples were examined by indirect fluorescent antibody test (IFAT). It was found a seroprevalence of 12.8%, considering titers >64. Therefore, the data reinforce the need for appropriate management of swine raising to minimize the risk of infection of pigs with T. gondii.

scholarly journals Comparison of Enzyme-Linked Immunosorbent Assay, Indirect Fluorescent Antibody Test, and Direct Agglutination Test for Detecting Toxoplasma Gondii Antibodies in Naturally Aborted Ovine Fetuses

1989 ◽  
Vol 1 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Sheryl L. Seefeldt ◽  
Clyde A. Kirkbride ◽  
Jitender P. Dubey
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Special Equipment

Results obtained in an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFA), and a modified direct agglutination test (MAT) for Toxoplasma gondii antibodies from examination of fetal fluids from 377 aborted ovine fetuses were compared. Sixty-seven samples were positive by MAT (titers 1:16 to > 1:65,536), 58 were positive by ELISA, and 62 were positive by immunoglobulin G-IFA. The MAT was preferred because it required less time, labor, and special equipment. It was simple to run, could be done on serum from any species without modification, and it was more effective than the IFA for detecting toxoplasma antibodies in severely autolyzed fetuses. No advantage was found in determining immunoglobulin M antibodies in ovine fetal sera.

scholarly journals Prevalence of Toxoplasma gondii indirect fluorescent antibodies in naturally- and experimentally-infected chickens (Gallus domesticus) in Thailand

2009 ◽  
Vol 54 (3) ◽  
Author(s):  
Kamlang Chumpolbanchorn ◽  
Pacharaporn Anankeatikul ◽  
Wantanee Ratanasak ◽  
Jitbanjong Wiengcharoen ◽  
R. Andrew Thompson ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Health Risks ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Gallus Domesticus ◽  
Indirect Fluorescent Antibody ◽  
Serum Samples ◽  
Public Health Risks ◽  
Potential Public Health

AbstractToxoplasma gondii infections in free-range (FR) chickens (Gallus domesticus) are potential public health risks. Antibodies for T. gondii were found in 194 out of 303 serum samples (64.03%) from FR chickens in Thailand tested by the indirect fluorescent antibody test (IFAT, 1:16). To verify the validity of serologic data in this survey, sera from chickens experimentally infected with the RH strain of T. gondii were tested by the IFAT. Antibodies against T. gondii were detected as early as 7 days p.i., peaked at 2 weeks, and then declined by 10 weeks p.i.

Comparison of indirect fluorescent antibody test and the modified agglutination test for the detection of Toxoplasma gondii antibodies in stray dogs from Southern Brazil

2016 ◽  
Vol 61 (4) ◽  
Author(s):  
Jonatas Campos de Almeida ◽  
Michelle Salmon Frehse ◽  
Italmar Teodorico Navarro ◽  
João Luis Garcia ◽  
Alexander Welker Biondo ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Southern Brazil ◽  
Stray Dogs ◽  
Modified Agglutination Test

AbstractThe aim of the present study was to determine the prevalence of antibodies to

Seroprevalence of Toxoplasma gondii and Neospora caninum infection of cats in Hungary

2008 ◽  
Vol 56 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Sándor Hornok ◽  
Renate Edelhofer ◽  
Anja Joachim ◽  
Róbert Farkas ◽  
Krisztián Berta ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Concurrent Infection ◽  
Indirect Fluorescent Antibody ◽  
Caninum Infection ◽  
Blood Samples ◽  
Feline Infectious Peritonitis

Blood samples were collected from 330 cats in Hungary in order to evaluate their seroconversion to Toxoplasma gondii and Neospora caninum using the indirect fluorescent antibody test (IFAT). The overall prevalence of toxoplasmosis was 47.6%, the prevalence being 22.4% among urban, 50% among suburban and 61.3% among rural animals. Significantly more cats had high IFAT titres (1:640 to 1:5120) in the countryside. Female cats were more frequently infected with T. gondii than males (53.3% vs. 39.3%), and seropositivity increased with the age of animals. The prevalence (0.6%) and titre (1:40) of antibodies to N. caninum was low. Sixty-two cats were also screened for seroconversion to feline infectious peritonitis (FIP) virus. Higher titres to T. gondii were more frequently detected among FIP-positive cats, but this difference was non-significant due to the small number of cats with concurrent infection.

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